The Antioxidant Effect of Rosmarinus officinalis L. Hydro Alcoholic Extract on Wheat

Using on a bigger scale in agriculture of the substances like pesticides which, besides, the benefit effects that exert on the cultures, present, more often than not, also a toxicity degree unusually high, due to the fact that these substances are absorbed at the different tissues and organs and, in the same time with the ingestion of the fruits and of the vegetables treated, these compounds get into the human organism and animal organism where pop up disequilibrium of metabolic reactions. On the other hand, the risen rema-nence of these substances in the soil determines perturbations of its quality through the straight effect on the microbial communities (1). The dinitrophenol (DNP) and its esters proved to be efficient like pesticides due to the multiple biological actions that manifests, their toxicity being particularly high, reason for what they are considered pollutant factors for human and its environment (2). In the last two years, the free radicals and the oxidative stress polarized the attention of researchers due to their involvement in the starting of a huge number of affections, a lot of researchers from medical and biologic domains orienting towards the decipherment of the mechanisms which are at the base of this process, taken into account the increasing importance of oxidative stress in multiple degenerative diseases and mental illness (3). The huge contrast between the vegetable potential of our country and the lower number of species valued in medical purpose on the strength of scientific fundamentation, impose the intensifica-tion of phytochemical researches in the aim of identifying some new sources of natural compounds , efficient from therapeutically point of view. The rosemary belongs to Lamiaceae family and exerts strongly antioxidant effects (4, 5) due to active principles that contains, of volatile oil type, phenolic compounds, flavonoides and di-terpenes (6). We tested the antioxidant capacity of Rosmarinus officinalis L. hydro-alcoholic extract in wheat seeds from Putna variety derived from the Research Resort and Agricultural Development Suceava. The caryopses were submitted to germination in lab conditions after a preliminary consecutive imbibition with DNP 10-3 M solution, followed by the rosemary hydro-alcoholic extract (RHE) in 0.5% concentration, obtained at cold with 70% ethanol in a 1:7 rapport. After 7 d from the debut of germination process were harvested the viable seedling and was determined the peroxidase's activity (POX) through o-dianisidine method, catalase's activity (CAT) through Sinha method